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In summary, accumulating evidence supports a critical role for ferroptosis in secondary injury after both TBI and SCI, driven by iron overload, lipid peroxidation, and dysregulated ferroptosis-associated proteins (114, 225235)

While the greater amount of cellular reduced GSH is found in the cytosol and mitochondria, the endoplasmic reticulum becomes a reservoir of small concentrations of the oxidized form of GSH (GSSG)

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The adaptor ligation step was performed as directed albeit with a custom pre-annealed oligo adaptor comprising of YL001 (/5Phos/GATCGGAAGAG/3ddC/) annealed to one of YL002:005 (AGCGGCAATTTCACACAGGACAAGCAGAAGACGGCATACGAGATNNNNNNNN AGTG GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC*T) where the 4-base underlined sequence serves as a variable barcode unique to each oligo